If using sensitive assays like FRET or FP, ensure the gain settings are not set too high. 5. Summary of Resources
The remains a sought‑after document because the instrument continues to perform well more than a decade after its discontinuation. While the official manual is not freely available, this article has collected a substantial amount of equivalent information from original Tecan press releases, installation guides, software reference manuals, and user‑generated content. By combining the hardware installation notes from the Freedom EVO service manual, the software instructions from the Magellan Reference Manual, and the community‑shared maintenance practices, you can safely operate and maintain your Safire 2.
Choose your detection mode (e.g., Fluorescence Intensity Top).
When you acquire the PDF, prioritize these sections to ensure the longevity of your equipment: tecan safire 2 manual pdf
The Safire 2 stands out due to its dual-monochromator design, which eliminates the need for expensive optical filters. Specification High-energy Xenon flash lamp Wavelength Selection Quadruple monochromators (2 excitation, 2 emission) Wavelength Range 230 nm to 850 nm (absorbance, fluorescence) Plate Formats 6-well up to 1536-well plates Detection Modes
Connect the unit to a grounded electrical outlet. Utilizing an Uninterruptible Power Supply (UPS) is highly recommended to protect the sensitive internal monochromators from power surges.
The safest and most comprehensive resource for instrument documentation is the official Tecan customer portal. Registered users can log in, navigate to the product archives, and download the full operator manual, software handbooks, and declaration of conformity documents. If using sensitive assays like FRET or FP,
: Typically operated using Magellan or i-control software for data acquisition and analysis.
Ensure the plate transport mechanism moves smoothly without jerking or catching. Common Troubleshooting Scenarios Potential Cause Recommended Action Initialization Error Plate carrier jammed or shipping locks not removed.
Select the exact plate manufacturer and well format (e.g., Greiner 96-well flat bottom) to ensure correct optical alignment. While the official manual is not freely available,
The installation section of the manual also warns that the reader must not be lifted by a single person; two persons are required due to the 40 kg weight.
Turn off power, manually clear obstruction, clean and lubricate carrier tracks. Contaminated microplate bottom or dust on internal lenses.
Loose interface cable or faulty COM port assignment in Windows Device Manager.